NEUROPROTECTIVE ROLE OF VITAMIN D3 ON GANGLION CELL-INNER PLEXIFORM LAYER THICKNESS IN NMDA-INDUCED RETINAL EXCITOTOXICITY MODEL OF GLAUCOMA (Experimental Study on Wistar Rats Glaucoma Model with N-Methyl-D-Aspartate induction)
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NMDA, Vitamin D3, GC-IPL Thickness, retinal ganglion cells, glaucoma
Abstract
Introduction & Objectives
Retinal ganglion cell (RGC) damage can affect the thinning of the GCC layers, which is the nerve
fiber layer, the ganglion cell layer and the inner plexiform layer. The loss of RGC and inner plexiform
layer (IPL) is strongly correlated with the loss of visual field defect. RGC and inner plexiform layer
are potential targets to evaluate the progression of glaucoma. Glaucoma treatment not only focus on
reducing IOP. Decreasing IOP according to the target pressure often can’t stop the process of RGC
damage and make it permanently and irreversible. Vitamin D3 is known as neuroprotector which has
the ability to protect RGC and prevent layer thinning of GC-IPL in retina. The aim of this study is to
determine the effect of oral Vitamin D3 on the GC- IPL layer thickness in retinal of Wistar rats with
NMDA-induced glaucoma model.
Methods
Glaucoma Wistar rats were divided into 2 groups. The treatment group was given oral Vitamin D3 at
a dose of 500 IU/kgBW/day for 10 weeks. GC-IPL layer thickness in retinal was examined by
Hematoxylin Eosin staining. Data were collected and processed then used with Independent T Test.
(significant p<0.05)
Results
The mean GC-IPL layer Thickness in the treatment group was 77,59 ± 15,15 ?m and 35,32 ± 4,04
?m in the control group. There was a significant different between two group ( p = <0,001 )
Conclusion
Vitamin D3 has a neuroprotective effect on ganglion cell-inner plexiform layer Wistar rats in NMDAinduced
excitotoxicity model of glaucoma
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Authors
Copyright (c) 2023 Ezra Margareth, Trilaksana Nugroho, Andhika Guna Dharma, Fifin L Rahmi, Maharani, Hermawan Istiadi
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