The Comparison of S100 A8/A9 Protein Receptor Expression between Progressive and Non-Progressive Pterygium
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Abstract
Background: S100 A8/A9 protein suggest have an activity at inflammation pathway caused fibroblast proliferation which is support the patofisiology teori of pterygium.
Methods: This research is a cross sectional study consists of 21 subject who suffered primary nasal or temporal pterygium. All subject divided into 2 grup, progressive dan non progressive pterygium with this following criteria: length of pterygium at the cornea, diameter ilots de Fuch, thickness of corpus compare with apex dan hiperemia of pterygium. The tissue of pterygium after excised were examined through histopatology and immunohistochemical with HE and reagent Mab 27E10 staining to see S100 A8/A9 protein reseptor expression. And than we calculate between staining cell and intencity of the brown-colour of reseptor that call staining Index to see the trend to proggressive pterygium.
Results: From 21 subject of study, amount of 11 subject (52,38%) progressive pterygium and 10 subject non progressive pterygium (47,62%). We found S100 A8/A9 protein reseptor expression at proggressive group were higher than non progressive group. Cut off point in staining index is 25 that show the pterygium has trend to be proggresive at that point or more. Histopatology examination there are strong correlation progresifity pterygium with squamous epithel prolifertion, cell inflamation, and vascular proliferation.
Conclusion: There is a significant differentiate in S100 A8/A9 protein receptor expression between progressive and non progressive pterygium.
Keywords: S100 A8/A9 protein, progressive pterygium, immunohistochemical
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References
2. Tasman, W. Jaeger, E A. Pathology of the Eye. Duane’s Ophthalmology. Lippincott William & Wilkins 2007:8
3. Ibrahim. Penilaian Progresifitas Pterygium Berdasarkan Pemeriksaan Kilnis dab Laboratoris. Bagian IK Mata FK Unsri/RSMH Palembang. 1998
4. Erry, et al. Distribusi dan Karakteristik Pterygium di Indonesia. BPPK. Menkes. Percetakan Negara
5. Daya Papalkar. Inflamation associated S100 protein in pterygium, tears, and uv irradiated murine corneas. Faculty of Medicine. University of New South Wales. 2010.
6. Khurana AK. Degenerative condition. In: Comprehensive ophthalmology. Fourth edition. New Age International Publisher. New Delhi 2007;80-2
7. Gebhardt C, et al. RAGE signaling sustains inflammation and promotes tumor development. 2008. J Exp Med 205:275-85.
8. Ando, R. Et al. Tissue factor expression in human pterygium. Mol Vis. 2011 Jan 8;17:63-9.
9. Kanski JJ. Pterygium. In: Clinical ophthalmology a systematic approach. Chapter 4. Butterworth Heinemann Elsevier:2007;242-5
10. Lin, Mol Vis 2013; 19:166-173. Serial Online. [Hiperlink] Available at. http://www.molvis.org/molvis/v19/166/mv-v19-166-f1.html
11. Dzunic, B. Et al. Analysis of pathohistological characteristics of pterygium. Bosnian Journal of Basic Medical Sciences 2010; 10 (4): 307-313
12. Karnen, G. Imunologi Dasar. FKUI. 2001:42
13. Chui J, Coroneo Minas. Ophthalmic pterygium: a stem cell disorder with premalignant features. The American Journal of Pathology. 2011:178(2):817-827
14. M T Coroneo. Pterygium as an early indicator of ultraviolet insolation: a hypothesis. British Journal of Ophthalmology. 1993;77:734-739
15. Detorakis E, Spandidos. Pathogenic mechanism and treatment option for ophthalmic pterygium: trends and perspective. International Journal of Molecular Medicine . 2009;23:439-447
16. Skuta GL, Cantor B, Weiss JS, et al. Surgery of the ocular surface. In: External eye disease. San Fransisco. 2012:sec 8;chapter 14; 391-3.
17. Skolnick C. Management of pterygium. Sec 2. Conjunctival surgery, p.1745-1761.
18. Tradjutrisno. Pterygium: degeneration, exuberant wound healing or benign neoplasm?. Universa Medicina; 2009;28(3);179-187.
19. Markowitz, J.Carson, WE. Review of S100 A9 Biology and Its Role in Cancer. Biochimica et Biophysica Acta .2013: 1835 :100-9
20. Nemeth, J. Angel, P. Hess, J. Dual Role of S100 A8 and A9 in Inflammation-Associated Cancer. Anti-Inflammatory & Anti-Allergy in Medicinal Chemistry. 2009:8:329-336
21. Dougan, M. Dranoff, G. Inciting Inflammation: The RAGE about Tumor Promotion. JEM. 2008:205:267-270
22. Robinson, M. et al. The S100 Family Heterodimer, MRP- 8/14, Binds with High Affinity to Heparin and Heparan Sulfate Glycosaminoglycans on Endothelial Cells. The Journal of Biological Chemistry 2002;277(5):p. 3658–3665
23. Nakatani, Y. et al. Regulation of S100A8/A9 (Calprotectin) Binding to Tumor Cells by Zinc Ion and Its Implication for Apoptosis-Inducing Activity. Mediators of Inflammation. 2005:5:p.280-292
24. Jharmarwala, M. Jhaveri, R. Pterygium: A New Surgical Technique. Journal of the Bombay Ophthalmologists’ Association. 2001:11:4
25. Standar pelayanan medis ilmu kesehatan mata. RS. Dr. M. Hoesin Palembang 2006:120-1