THE EXPRESSION OF ALPHA-SMOOTH MUSCLE ACTIN IN CONTRACTED SOCKET

Theresia Dwiamelia; Datu Respatika; Irene Titin Darajati; Banu Aji Dibyasakti; Purjanto Tepo Utomo; Agus Supartoto

doi:10.35749/k6np2295

Oncology and Plastic Reconstructive

Theresia Dwiamelia ⁽¹⁾ , Datu Respatika ⁽²⁾ , Irene Titin Darajati ⁽³⁾ , Banu Aji Dibyasakti ⁽⁴⁾ , Purjanto Tepo Utomo ⁽⁵⁾ , Agus Supartoto ⁽⁶⁾

(1) Department of Ophthalmology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Sardjito General Hospital, Yogyakarta , Indonesia

(2) Department of Ophthalmology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Sardjito General Hospital, Yogyakarta , Indonesia

(3) Department of Ophthalmology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Sardjito General Hospital, Yogyakarta , Indonesia

(4) Department of Ophthalmology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Sardjito General Hospital, Yogyakarta , Indonesia

(5) Department of Ophthalmology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Sardjito General Hospital, Yogyakarta , Indonesia

(6) Department of Ophthalmology, Faculty of Medicine, Public Health and Nursing Gadjah Mada University, Sardjito General Hospital, Yogyakarta , Indonesia

https://doi.org/10.35749/k6np2295

Issue
Vol. 49 No. S1 (2023): Supplement Edition

Submitted
August 27, 2023

Published
February 12, 2024

Keywords:

myofibroblast, alpha-SMA, contracted socket

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Abstract

Purpose: A contracted socket is considered a common complication in anophthalmic patients, with the resulting poor fitting of an ocular prosthesis. In severe cases where patients have had multiple previous reconstructive attempts, further socket contraction may result from fibrosis, leading to eventual surgical failure. Alpha-smooth muscle actin (?-SMA) is often used to identify pathologic fibroblasts. It may serve as a reliable marker for myofibroblast cells. The aim of the study was to find ?-SMA protein expression in myofibroblasts derived from orbital socket contracture.

Methods: In this study, we observed five patients with contracted sockets who underwent fornicoplasty and buccal graft surgery from October 2022 to February 2023. Tissue samples were taken from the conjunctival fornix area during the surgery and then examined for ?-SMA expression.

Result: In this study, there were 2 out of 5 (40%) positive ?-SMA expressions with history of recurrent contraction, 2 (40%) negative expressions with no history of recurrent contraction, and 1 result that could not be assessed due to the limited number of samples containing fibroblasts.

Conclusion: ?-SMA levels can be detected in patients with contracted sockets. Patients with a history of recurrently contracted sockets have a tendency to express positive ?-SMA. This may allow for the need for the administration of antimetabolites in patients with recurrent contracted sockets to avoid recurrence of this contracted socket.

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