THE EFFECT OF ORAL VITAMIN D ON TUMOR NECROSIS FACTOR-? EXPRESSION IN TRABECULAR MESHWORK OF WISTAR GLAUCOMA MODEL Oral Presentation - Experimental Study - Resident
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Vitamin D, TNF-?, trabecular meshwork, glaucoma
Abstract
Introduction & Objectives
Elevated intraocular pressure (IOP) is a risk factor for the development and progression of glaucoma
that can induces inflammation in the trabecular meshwork through the production of proinflammatory
cytokines such as TNF-?. Inflammation induce mechanical stress and changes in the
extracellular matrix in the trabecular meshwork, resulting in hypertonicity and resistance to
aqueous humor outflow. Vitamin D can modulate cytokine production by suppressing TH1 cell
expression and inducing TH2 cell expression. The production of pro-inflammatory cytokines can be
suppressed by the presence of vitamin D.
Methods
An experimental study of 30 wistar glaucoma model were divided into 3 groups, non treatment
group ( A and B) and the oral vitamin D (1200 IU/kg/day) group (C) for 14 days. TNF-? expressions
in trabecular meshwork were measured by immunohistochemical staining. Group A was measured
on day 3, while group B and C were measured on day 14. The differences between the three groups
were analyzed statistically using one way ANOVA test. Significantly different if the p<0.05.
Results
The mean expression of TNF- ? in the trabecular meshwork of wistar glaucoma model in group A, B,
and C was 22,2 ± 2,51%, 39,1 ± 3.98%, 34.2 ± 3,19 % respectively. There was significantly
difference in TNF-? expressions between the three groups (p<0.001).
Conclusion
Vitamin D per oral can decrease TNF-? expression in the trabecular meshwork of wistar glaucoma
model on day 14 . Studies in wistar and humans are still needed to assess the effect of vitamin D on
others pro-inflammatory cytokines in glaucoma.
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