Efficacy of Sambiloto Extracts, Andrographis paniculate, (Burm. F) in Inhibiting Diabetic Retinopathy Progression: An in Vivo Study Oral Presentation - Experimental Study - Resident
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Andrographis paniculata, Antioxidants, Diabetic Retinopathy, Plant Extracts, Retinal Vessels
Abstract
Introduction & Objectives
Diabetic retinopathy (DR) is one of diabetes mellitus complication and occurred in retinal
microvascular. This study was aimed to investigate the efficacy of Sambiloto, Andrographis
paniculate (A. paniculata) extract on glycemic profile, antioxidant and inflammatory cytokine
parameters in diabetic rats, and phytochemical analysis of A. paniculata.
Methods
A. paniculata extract (APE) was carried out by maceration with ethanol. Diabetes mellitus in Wistar
male rats was induced with streptozotocin. Retinal vessel diameters were estimated using a method
by Vucetic. Inflammatory cytokine and antioxidant parameters were evaluated in retinal tissue. The
alkaloid and flavonoid contents in extract were analyzed using thin layer chromatography method.
Results
Funduscopic examination presented some changes in the diameter of the blood vessels. The vessel
diameter in the diabetic retinopathy group with APE in concentration of 100 and 200 mg/kg BW
groups was significantly lower than in the DR group (p<0.05). The administration of APE in dosages
of 100 and 200 mg/kg BW showed reduced glutathione, SOD, and catalase levels compared to the
DR group (p<0.05).
Conclusion
A. paniculata extract doses of 100 and 200 mg/kg BW improved diabetic retinopathy in rats through
hypoglycemic effects, antioxidant effects, and anti-inflammatory mechanisms.
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Copyright (c) 2023 Muhammad Apriliandy Shariff, Ramzi Amin, AK. Ansyori
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