Relationship between Serum Homocysteine Levels and Manganese Superoxide Dismutase (MnSOD) on Retinal Nerve Fiber Layer (RNFL) Thickness in Diabetic Retinopathy and Non-Diabetic Retinopathy Patients Oral Presentation - Observational Study - Resident
Article Sidebar
-
Diabetic Retinopathy, Homocysteine, MnSOD, RNFL
Abstract
Introduction & Objectives
Diabetic retinopathy is a visual disorder caused by abnormalities in the retina, where there is a
progressive microangiopathy characterized by damage and blockage of fine blood vessels, resulting
in impaired nutrition of the retina. This study is to find out the relationship between serum
homocysteine levels and MnSOD on retinal nerve fiber layer (RNFL) thickness in diabetic
retinopathy and non-diabetic retinopathy patients.
Methods
This study is an analytic observational case-control study with a total of 31 subjects for each group.
3cc of venous blood samples were taken to assess the serum levels of homocysteine and MnSOD and
then analyzed by ELISA method in the integrated laboratory of the USU Medical Faculty.
Results
There was a significant difference in Avg RNFL and Nasal RNFL thickness between the two groups
(p=0.015) with the mean Avg RNFL thickness being 100.94 (SD=17.04) and the mean RNFL nasal
thickness being 90.16 (SD=33.68). The highest MnSOD levels were found in non-diabetic
retinopathy subjects with a mean of 142.77 (SD=101.77). There was no significant relationship
between homocysteine and thickness of superior RNFL (p=0.346), inferior RNFL (p=0.292),
temporal RNFL (p=0.109), nasal RNFL (p=0.190). However, a significant association was found
between homocysteine and Avg RNFL (p=0.007).
Conclusion
In the group of cases of diabetic retinopathy, a significant relationship was found between Hcy and
Avg RNFL (p=0.007). An increase in serum homocysteine levels will be followed by a decrease in the
thickness of the Avg RNFL.
Full text article
References
American Academy of Ophthalmology. Section 12: Retina and Vitreous BCSC. Basic and Clinical Science Course. In: Retinal Vascular Disease: Diabetic Retinopathy. USA: American Academy of Ophthalmology; 2020- 2021; p.122-49.
Srivastav K, Saxena S, Mahdi AA, Shukla RK, Meyer CH, Akduman L, et al. Increased Serum Level of Homocysteine Correlates with Retinal Nerve Fiber Layer Thinning in Diabetic Retinopathy. Molecular Vision. 2016 December; 22:1352-60.
Retina/ Vitreous Preferred Practice Patterns. Diabetic Retinopathy PPP. San Fransisco: American Academy of Ophthalmology Retina/ Vitreous Panel. 2019.
Sasongko MB, Widyaputri F, Agni AN. Prevalence of Diabetic Retinopathy and Blindness in Adults with Type 2 Diabetes. Indonesia: American Journal of Ophthalmology. 2019; 181: p.79-87
Xu Y, Wang A, Lin X, dkk. Global burden and gender disparity of vision loss associated with diabetes retinopathy. Acta Ophthalmologica Scandinavica Foundation. 2020. p.1-10.
Li M, Wang Y, Liu Z, dkk. Females with Type 2 Diabetes Mellitus Are Prone to Diabetic Retinopathy: A Twelve-Province Cross-Sectional Study in China. Hindawi J Diab Res. 2020. p.1-9
BCSC. Basic and Clinical Science Course. In: Retinal Vascular Disease: Diabetic Retinopathy. USA: American Academy of Ophthalmology; 2020- 2021; p.122-49.
Srivastav K, Saxena S, Mahdi AA, Shukla RK, Meyer CH, Akduman L, et al. Increased Serum Level of Homocysteine Correlates with Retinal Nerve Fiber Layer Thinning in Diabetic Retinopathy. Molecular Vision. 2016 December; 22:1352-60
Teo ZL, Tam YC, Yu M. Global Prevalence of Diabetic Retinopathy and Projection of Burden through 2045. American Academy of Ophthalmology. J ophtha. 2021. p.1-12
Hertapanndika IN, Sutyawan IWE, Triningrat AAMP. Profil Retinopati Diabetik di Divisi VitreoRetina Ruma Sakit Umum Pusat Sanglah Denpasar 1 Januari–30 Juni 2015. Jur Med Udayana. Maret, 2020; Vol. 9 No.3: p.1-7.
El-Hifnawy MA, Sabry KM, Gomaa AR, Hassan TA. Effect of diabetic retinopathy on retinal nerve fiber layer thickness. 2016. Del J Ophtha. Vol.17(3); p.162.
Ho H, Tham YC, Chee ML, Shi Y, Tan NY, Wong KH, Cheng CY. Retinal Nerve Fiber Layer Thickness in A Multiethnic Normal Asian Population: The Singapore Epidemiology of Eye Diseases Study. 2019. Ophthalmology. Vol. 126(5); p.702-711.
Shi R, Guo Z, Wang F, Li R, Zhao L, Lin R. Alterations in Retinal Nerve Fiber Layer Thickness in Early Stages of Diabetic Retinopathy and Potential Risk Factors. Curr eye res. 2017 November; 09: p.
Vujosevic S, Midena E. Retinal layers changes in human preclinical and early clinical diabetic retinopathy support early retinal neuronal and Müller cells alterations. 2013. J Diab Res. 2013; 8p.
Srivastav K, Saxena S, Mahdi AA, Shukla RK, Meyer CH, Akduman L, et al. Increased Serum Level of Homocysteine Correlates with Retinal Nerve Fiber Layer Thinning in Diabetic Retinopathy. Molecular Vision. 2016 December; 22:1352-60.
Lei X, Zeng G, Zhang Y, Li Q, Zhang J, Bai Z, et al. Association Between Homocysteine Level and the Risk of Diabetic Retinopathy: A Systematic Review and meta-analysis. Diabetol Metab Syndr. 2018; 10: p.61.
Muzurovi? E, Kraljevi? I, Solak M, Dragni? S, Mikhailidis DP. Homocysteine and diabetes: role in macrovascular and microvascular complications. 2021. Journal of Diabetes and its Complications. Vol. 35(3)
Kowluru RA. Diabetic Retinopathy: Mitochondria Caught in A Muddle of Homocysteine. J Clin Med. 2020; 9: p.3019.
Kowluru RA, Mohammad G, Sahajpal N. Faulty Homocysteine Recycling in Diabetic Retinopathy. Eye and vision. 2020; 7: p.4
Qamar MU, Gill M, Qureshi HJ, Aslam MS, Fazal A, Masud S. Association of Serum C-Reactive Protein (CRP), Cystatin C and Homocysteine with Diabetic Retinopathy in Patiens of Type 2 Diabetes Mellitus. J Akh Sae Med Dent Coll. 2020 Jan -Mar; 02(01).
Chatterjee S, Chakraborti S. Can serum MDA:SOD Ratio Predict Risk of Retinopathy in Type 2 Diabetes Mellitus?. Int J Res Rev. 2020 January; 7: 4p
Brzovi?-Šari? V, Landeka I, Šari? B, Barberi? M, Andrijaševi? L, Cerovski B, Oršoli? N, ?iki? D. Levels of selected oxidative stress markers in the vitreous and serum of diabetic retinopathy patients. Mol Vis. 2015 Jun 12;21:p.649-64.
Huma K, Mehboob K, Rashid A, Majeed A. A Comparative Analysis of Superoxide Dismutase 1 level in Diabetics with and without Neuropathy. J Col Phys Surg. 2021; 31(7): p.765-69.
Fei Z, Gao W, Xu X, Sheng H, Qu S, Cui R. Serum Superoxide Dismutase Activity: A Sensitive, Convenient, and Economical Indicator Associated with the Prevalence of Chronic Type 2 Diabetic Complications, Especially in Men. Free Rad Res. 2021; 55(3): p.275-81.
Authors
Copyright (c) 2023 Rini Nurrakhmah, Delfi, Rodiah Rahmawaty Lubis, Vanda Virgayanti
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.